Invivo Adaptogenic Activity Assessment of Sambucus nigra and Streblus asper: Standardization of Polyherbal Syrup, Investigation of Quercetin and Kempherol

Author(s): Sachin Neekhra, Alok Kumar Shukla, Shravan Kumar Paswan, Shikhar Verma and Himani Awasthi

Abstract:

People’s concern in traditional medicine has increased recently; however, customary herbal medicines need to be converted into a modern system of medicine to increase patient recognition. Objective - The present work includes the fabrication and characterization of polyherbal syrup with hydroalcoholic extractof Sambucus nigra and Streblus asper leaves. Methods - Physicochemical analysis of selected crude drug was done. Various physicochemical evaluations of polyherbal syrup such as pH, density, specific gravity, viscosity, refractive index and accelerated stability testing were determined. The qualitative determination of a flavonoidal biomarker (Quercetin and Kaempferol) was performed via HPTLC. Antistress activity was assessed using foot-shock induced stress model. Twenty albino rats (5 mice in each group) were grouped into normal control, stress control, polyherbal syrup (200 mg/kg) and diazepam (1 mg/kg). Polyherbal syrup treatment was given orally for 2 weeks with prior use of foot shock induced stress. Results - The study given above shows result data which are obtained from physio-chemical analysis of selected crude herbs for syrup preparation showed physicochemical characters, accelerated stability testing of polyherbal syrup was validated & standardised as per Ayurvedic pharmacopoeia limit. A significant variation was found in number of mountings, duration of immobility and active avoidance response in foot shock induced stress as compared to stress control group. The research data approved that polyherbal syrup showed significant anti-stress potential which attributes to the explored biomarkers (Quercetin and Kaempferol) and the stability studies of standardized formulation justify its shelf life.

Keywords: Anti-stress activity, Kaempferol, Polyherbal syrup, Quercetin, Sambucus nigra, Streblus asper

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