Development and Evaluation of Hydroalcoholic Extract of Alhagi Camelorum as NDDS

Author(s): Rishi Kant Tripathi, Dr. Anuj Kumar Sharma and Dr. Pritt Verma

Abstract:

The extract of Alhagi camelorum (AC) has poor solubility and bioavailability. The present work aimed to develop a AC loaded liposome to improve the solubility and bioavailability. A liposome was prepared using thin film hydration method, and the optimized liposomal formulation was prepared using soya phosphatidylcholine (PC) and cholesterol (CH) and AC in the molar ratio (7:3:5). Formulation optimization was done using central composite design of response surface methodology. Based on preliminary experiment, the three independent variables X1, X2, X3 where, sonication time (X1; mins), ratio of Phospholipid: Cholesterol (X2; mg), Temperature (X3; degree) and dependent variables are Y1, Y2 where, Particle size (Y1; nm), Entrapment efficiency (Y2; %). In vitro drug release of AC extract and AC loaded liposome were assessed and found that 49.45 % of extract from liposomes was released at pH 7.4 within 48 hours. The AC loaded liposome had particle size (101.2±2.19). The drug encapsulation efficiency of AC loaded liposome was 75%. The internal morphology of optimized liposomal formulation was seen by transmission electron microscopy and the surface morphology was done by scanning electron microscopy. This confirmed the usefulness of the liposomal delivery system for to improve solubility and bioavailability.

Keywords: Alhagi Camelorum, Novel Drug Delivery System, Liposomes, Particle Size, Entrapment Efficiency

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